Regulatory

Part:BBa_K740000:Design

Designed by: Lei Wei   Group: iGEM12_Tsinghua-A   (2012-09-23)


Constitutive promoter flanked by two loxP sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 41
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used 3 oligos to synthesize it.


Source

It was synthesized based on the sequence of J23115 and loxP site.

References